How ultraviolet–visible spectroscopy works
In short: A UV–Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet and visible light absorbed by a sample.
1. The process involves the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum. 2. Most molecules and ions absorb energy in the ultraviolet or visible range, acting as chromophores. 3. The UV–Vis spectrophotometer measures the amount of ultraviolet and visible light that is absorbed by a sample. 4. Parameters of interest include absorbance, transmittance, or reflectance, and their change with time.
Sources: Wikipedia, “Ultraviolet–visible spectroscopy (Wikipedia)” (CC BY-SA 4.0). Illustrative, not to scale.
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